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Chromatographic conditions (a) Use a stainless steel column (10 cm 3.0 mm) packed with phenylsilyl silica gel for chromatography (5 m) (Inertsil Phenyl is suitable)

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To separate MCF derivatives from the reaction mixture, 400 L chloroform was added and vigorously mixed for 10 s followed by the addition of 400 L sodium bicarbonate (50 mM), and mixing for an additional 10 s